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7.Preparation of yeast recombinant expression of mendong insulin
Source: | Author:pro954f45 | Published time: 2018-10-17 | 178 Views | Share:
    Preparation of yeast recombinant expression of mendong insulin. The invention discloses a preparation method of pichia pastoris recombinant expression of mendong insulin. Specifically, it includes the process of high-efficient secretion and expression of recombinant human pyrimidine in yeast, single enzyme digestion of lysyl endopeptidase to obtain b30-deficient pyrimidine, coupled threonine ester, deprotection, reverse phase purification and crystallization. The method is more suitable for industrialized preparation of recombinant mendong insulin.